1H NMR-based investigation of metabolic response to electro-acupuncture stimulation

Caigui Lin, Zhiliang Wei, Kian Kai Cheng, Jingjing Xu, Guiping Shen, Chang She, Huan Zhong, Xiaorong Chang, Jiyang Dong

Research output: Research - peer-reviewArticle

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Abstract

Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian specificity using proton nuclear magnetic resonance (1H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. 1H-NMR spectra datasets of serum, urine, cortex, and stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS meridians. These results validate the hypothesis that meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture.

LanguageEnglish
Article number6820
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2017

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Meridians
Acupuncture
Foot
Acupuncture Points
Stomach
Gallbladder
Acupuncture Therapy
Metabolomics
Therapeutics
Tissue Extracts
Metabolome
Chinese Traditional Medicine
Choline
Needles
Sprague Dawley Rats
Protons
Esters
Magnetic Resonance Spectroscopy
Multivariate Analysis
Urine

ASJC Scopus subject areas

  • General

Cite this

1H NMR-based investigation of metabolic response to electro-acupuncture stimulation. / Lin, Caigui; Wei, Zhiliang; Cheng, Kian Kai; Xu, Jingjing; Shen, Guiping; She, Chang; Zhong, Huan; Chang, Xiaorong; Dong, Jiyang.

In: Scientific Reports, Vol. 7, No. 1, 6820, 01.12.2017.

Research output: Research - peer-reviewArticle

Lin, C, Wei, Z, Cheng, KK, Xu, J, Shen, G, She, C, Zhong, H, Chang, X & Dong, J 2017, '1H NMR-based investigation of metabolic response to electro-acupuncture stimulation' Scientific Reports, vol 7, no. 1, 6820. DOI: 10.1038/s41598-017-07306-5
Lin, Caigui ; Wei, Zhiliang ; Cheng, Kian Kai ; Xu, Jingjing ; Shen, Guiping ; She, Chang ; Zhong, Huan ; Chang, Xiaorong ; Dong, Jiyang. / 1H NMR-based investigation of metabolic response to electro-acupuncture stimulation. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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