Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles

Nur Atiqah Lyana Binti Nor Ashikin, Mohd Khairul Hakimi Bin Abdul Wahab, Rosli Md Illias, Siti Fatimah Zaharah Mohd Fuzi

Research output: ResearchChapter

Abstract

Immobilisation process can be applied for both whole cells and enzymes to optimise the operational performance system for industrial applications. A successful immobilisation process leads to the development of economically and ecologically available biocatalyst such as xylanase. However cell lysis becomes one of the biggest problems in the enzyme excretion when E. coli is used as a host. In this study, the effects of different nanoparticles on xylanase excretion and cell lysis of immobilized E. coli were examined. For protein expression, the cells were cultured in various immobilized matrices on graphene oxide treated, graphene oxide untreated, carbon nanotube treated and carbon nanotube untreated with 100 mg/mL IPTG concentrations at 30 °C temperature and 200 rpm agitation rate for 24 h. The immobilised cells demonstrated a 7 % increase in xylanase excretion and a 39 % reduction of cell lysis compared with free cells using untreated graphene oxide. Consequently, the immobilisation of E. coli using nanoparticles was verified to increase xylanase excretion and cell stability.

LanguageEnglish
Title of host publicationChemical Engineering Transactions
PublisherItalian Association of Chemical Engineering - AIDIC
Pages1825-1830
Number of pages6
Volume56
ISBN (Electronic)9788895608471
DOIs
StatePublished - 2017

Publication series

NameChemical Engineering Transactions
Volume56
ISSN (Electronic)2283-9216

Fingerprint

Graphite
Oxides
Escherichia coli
Nanoparticles
Enzymes
Graphene
Carbon Nanotubes
Carbon nanotubes
Isopropyl Thiogalactoside
Industrial applications
Cells
Proteins
Temperature
Biocatalysts

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Nor Ashikin, N. A. L. B., Wahab, M. K. H. B. A., Illias, R. M., & Fuzi, S. F. Z. M. (2017). Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles. In Chemical Engineering Transactions (Vol. 56, pp. 1825-1830). (Chemical Engineering Transactions; Vol. 56). Italian Association of Chemical Engineering - AIDIC. DOI: 10.3303/CET1756305

Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles. / Nor Ashikin, Nur Atiqah Lyana Binti; Wahab, Mohd Khairul Hakimi Bin Abdul; Illias, Rosli Md; Fuzi, Siti Fatimah Zaharah Mohd.

Chemical Engineering Transactions. Vol. 56 Italian Association of Chemical Engineering - AIDIC, 2017. p. 1825-1830 (Chemical Engineering Transactions; Vol. 56).

Research output: ResearchChapter

Nor Ashikin, NALB, Wahab, MKHBA, Illias, RM & Fuzi, SFZM 2017, Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles. in Chemical Engineering Transactions. vol. 56, Chemical Engineering Transactions, vol. 56, Italian Association of Chemical Engineering - AIDIC, pp. 1825-1830. DOI: 10.3303/CET1756305
Nor Ashikin NALB, Wahab MKHBA, Illias RM, Fuzi SFZM. Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles. In Chemical Engineering Transactions. Vol. 56. Italian Association of Chemical Engineering - AIDIC. 2017. p. 1825-1830. (Chemical Engineering Transactions). Available from, DOI: 10.3303/CET1756305
Nor Ashikin, Nur Atiqah Lyana Binti ; Wahab, Mohd Khairul Hakimi Bin Abdul ; Illias, Rosli Md ; Fuzi, Siti Fatimah Zaharah Mohd. / Comparison of thermostable xylanase production by Escherichia coli immobilised onto different nanoparticles. Chemical Engineering Transactions. Vol. 56 Italian Association of Chemical Engineering - AIDIC, 2017. pp. 1825-1830 (Chemical Engineering Transactions).
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