Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris

M. Md Tab, N. H F Hashim, F. D. Abu Bakar, R. Illias, N. Najimudin, N. M. Mahadi, A. M A Murad

Research output: Research - peer-reviewArticle

Abstract

Ice recrystallization during thawing post-cryopreservation results in extensive cellular damage and ultimately leads to cell death and reduced cell viability. Antifreeze proteins (AFPs) are a group of proteins that allow organisms to survive in subzero environments. These proteins have thermal hysteresis and ice recrystallization inhibitory activities. In this present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Antarctic yeast, Glaciozyma antarctica, as a recrystallization inhibitor (RI) of ice growth and assessed its application as a cryopreservative of the fungal cutinase enzyme against freeze-thaw cycles. Recombinant Afp1 from G. antarctica, a psychrophilic yeast, has been produced in a methylotrophic yeast, Pichia pastoris, system that results in the expression of a hyper-glycoprotein (~55 kDa). Recombinant Afp1 exhibits antifreeze functions: thermal hysteresis (TH) and recrystallization inhibition where the highest TH values recorded for ~0.5°C at 10 mg/mL. The cryoprotective effects of Afp1 on purified recombinant cutinase showed that Afp1 can retain enzymatic activity up to ~20% when subjected to several cycles of freeze thawing. These findings indicate that Afp1 might act as a cryoprotective agent and thus, has great potential in biotechnology applications.

LanguageEnglish
Pages213-218
Number of pages6
JournalMalaysian Applied Biology
Volume46
Issue number1
StatePublished - 1 Mar 2017

Fingerprint

Pichia pastoris
hysteresis
recombinant proteins
Antarctica
ice
yeasts
heat
antifreeze proteins
Antifreeze Proteins
Pichia
Ice
Recombinant Proteins
Yeasts
cutinase
thawing
proteins
Cryoprotective Agents
Hot Temperature
freeze-thaw cycles
cryopreservation

Keywords

  • Antifreeze protein
  • Cryoprotective
  • Glaciozyma antarctica
  • Recrystallization inhibition

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris. / Md Tab, M.; Hashim, N. H F; Abu Bakar, F. D.; Illias, R.; Najimudin, N.; Mahadi, N. M.; Murad, A. M A.

In: Malaysian Applied Biology, Vol. 46, No. 1, 01.03.2017, p. 213-218.

Research output: Research - peer-reviewArticle

Md Tab, M. ; Hashim, N. H F ; Abu Bakar, F. D. ; Illias, R. ; Najimudin, N. ; Mahadi, N. M. ; Murad, A. M A. / Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris. In: Malaysian Applied Biology. 2017 ; Vol. 46, No. 1. pp. 213-218
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