Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches

Doris Quay Huai Xia, Yong Hui Yee, Rosli Md Illias, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abdul Murad

Research output: Research - peer-reviewArticle

Abstract

The potential for recombinant cellulases from fungi to hydrolyze oil palm empty fruit bunches (OPEFB) into simple sugars was investigated. A mixture of two recombinant enzymes consisting of a cellobiohydrolase from Trichoderma reesei and an endoglucanase from Aspergillus niger, was evaluated for OPEFB hydrolysis. The development of A. niger endoglucanase has been described previously. In this work, the development of a recombinant cellobiohydrolase and the activity of this enzyme mixture towards OPEFB hydrolysis were described. To obtain recombinant cellobiohydrolase, the cDNA encoding for cellobiohydrolase, cbhII, was isolated from the T. reesei strain M5, and the gene was expressed in the methylotrophic yeast, Pichia pastoris. Partially purified CbhII demonstrated optimum activity at 50°C and pH 5.0. This enzyme was shown to hydrolyze Avicel at a concentration of 0.486 U/mg under optimum conditions. Hydrolysis of pretreated OPEFB using CbhII and endoglucanase EglA showed that the total reducing sugar produced was higher in the enzyme mixture reaction than were produced in an individual enzyme reaction. Addition of β-glucosidase and an enzyme cofactor significantly increased enzyme activity and OPEFB cellulose hydrolysis. This study demonstrated the capability of an enzyme mixture to produce sugars from oil palm lignocellulosic waste.

LanguageEnglish
Pages11-19
Number of pages9
JournalMalaysian Applied Biology
Volume46
Issue number2
StatePublished - 2017

Fingerprint

cellulose 1,4-beta-cellobiosidase
Trichoderma reesei
Elaeis guineensis
endo-1,4-beta-glucanase
fruits
Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Cellulase
Fruit
Oils
Enzymes
enzymes
hydrolysis
Hydrolysis
Aspergillus niger
enzyme activity
sugars
Cellulose
lignocellulosic wastes
Pichia pastoris

Keywords

  • Cellobiohydrolase
  • Enzyme mixture
  • Oil palm empty fruit bunch
  • Trichoderma reesei

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches. / Xia, Doris Quay Huai; Yee, Yong Hui; Illias, Rosli Md; Mahadi, Nor Muhammad; Bakar, Farah Diba Abu; Murad, Abdul Munir Abdul.

In: Malaysian Applied Biology, Vol. 46, No. 2, 2017, p. 11-19.

Research output: Research - peer-reviewArticle

Xia, Doris Quay Huai ; Yee, Yong Hui ; Illias, Rosli Md ; Mahadi, Nor Muhammad ; Bakar, Farah Diba Abu ; Murad, Abdul Munir Abdul. / Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches. In: Malaysian Applied Biology. 2017 ; Vol. 46, No. 2. pp. 11-19
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