Bioassay-guided isolation of antioxidants and α-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)

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Abstract

Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time.

LanguageEnglish
Pages406-410
Number of pages5
JournalRecords of Natural Products
Volume11
Issue number4
StatePublished - 2017

Fingerprint

Cassia
Quercetin
Fabaceae
Biological Assay
Antioxidants
Glycoside Hydrolase Inhibitors
Bioassay
alpha-glucosidase
bioassays
antioxidants
quercetin
alpha-Glucosidases
3,3',4,5'-tetrahydroxystilbene
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
ethyl acetate
Scavenging
Emodin
Acarbose
Butylated Hydroxytoluene
Silica Gel

Keywords

  • Antioxidant
  • Cassia sieberiana
  • Phytochemicals
  • α-glucosidase

ASJC Scopus subject areas

  • Pharmacology
  • Plant Science
  • Drug Discovery
  • Organic Chemistry

Cite this

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title = "Bioassay-guided isolation of antioxidants and α-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)",
abstract = "Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time.",
keywords = "Antioxidant, Cassia sieberiana, Phytochemicals, α-glucosidase",
author = "Saidu Jibril and Sirat, {Hasnah Mohd} and Norazah Basar",
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AU - Jibril,Saidu

AU - Sirat,Hasnah Mohd

AU - Basar,Norazah

PY - 2017

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N2 - Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time.

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