Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples

Nyuk Ting Ng, Mohd Marsin Sanagi, Wan Nazihah Wan Ibrahim, Wan Aini Wan Ibrahim

Research output: Contribution to journalArticle

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Abstract

Agarose-chitosan-immobilized octadecylsilyl-silica (C18) film micro-solid phase extraction (μSPE) was developed and applied for the determination of phenanthrene (PHE) and pyrene (PYR) in chrysanthemum tea samples using high performance liquid chromatography-ultraviolet detection (HPLC-UV). The film of blended agarose and chitosan allows good dispersion of C18, prevents the leaching of C18 during application and enhances the film mechanical stability. Important μSPE parameters were optimized including amount of sorbent loading, extraction time, desorption solvent and desorption time. The matrix match calibration curves showed good linearity (r ⩾ 0.994) over a concentration range of 1–500 ppb. Under the optimized conditions, the proposed method showed good limits of detection (0.549–0.673 ppb), good analyte recoveries (100.8–105.99%) and good reproducibilities (RSDs ⩽ 13.53%, n = 3) with preconcentration factors of 4 and 72 for PHE and PYR, respectively.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalFood Chemistry
Volume222
DOIs
StatePublished - 1 May 2017

Fingerprint

phenanthrene
pyrene (hydrocarbon)
chitosan
agarose
Chrysanthemum
Chitosan
Tea
Sepharose
High Pressure Liquid Chromatography
Arthroscopy
solid phase extraction
desorption
tea
high performance liquid chromatography
sampling
Silicon Dioxide
Calibration
Limit of Detection
Butylene Glycols
Defecation

Keywords

  • Agarose-chitosan-immobilized C adsorbent
  • Chrysanthemum tea
  • Micro-solid phase extraction
  • Phenanthrene
  • Pyrene

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples. / Ng, Nyuk Ting; Sanagi, Mohd Marsin; Wan Ibrahim, Wan Nazihah; Wan Ibrahim, Wan Aini.

In: Food Chemistry, Vol. 222, 01.05.2017, p. 28-34.

Research output: Contribution to journalArticle

Ng, Nyuk Ting; Sanagi, Mohd Marsin; Wan Ibrahim, Wan Nazihah; Wan Ibrahim, Wan Aini / Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples.

In: Food Chemistry, Vol. 222, 01.05.2017, p. 28-34.

Research output: Contribution to journalArticle

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